Plaque Formation and Isolation of Pure Lines with Poliomyelitis Viruses

The Formation of Plaques by African Horse Sickness Viruses and Factors Affecting Plaque Size

Construction of Various γ34.5 Deleted Fluorescent-Expressing Oncolytic herpes Simplex type 1 (oHSV) for Generation and Isolation of HSV-Based Vectors

Background: Oncolytic herpes simplex virus (oHSV)-based vectors lacking γ34.5 gene, are considered as ideal templates to construct efficient vectors for (targeted) cancer gene therapy. Herein, we reported the construction of three single/dually-flourescence labeled and γ34.5-deleted, recombinant HSV-1 vectors for rapid generation and easy selection/isolation of different HSV-Based v...

Evaluating of Iran Razi Khedmati Bovine Kidney (IRKHBK) Cell Line for Isolation and ‎Propagation of Viruses

Abstract Background and Aims: To identify and isolation of viruses a susceptible cell is need which can be primary or line. The use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. IRKHBK cell line was originated from primary cells of bovine kidney by continuous passage. The aim of this study was to introduce the IRKHBK cell ...

Plaque formation of LaSota pathogenic strain of Newcastle disease virus adapted in chick embryo fibroblast cells

In order to adapt LaSota strain of Newcastle disease virus (NDV) on chick embryo fibroblast (CEF) cells, 0.1 ml from LaSota vaccine produced in Razi vaccine and serum research institute inoculated to the CEF cells and was passaged five times in the CEF grown in Eagle’s minimum essential medium (MEM). First to third passages were blind but in fourth passage cytopathic effect of virus was observe...

Poliomyelitis Viruses in Tissue Culture 5

Tissue culture inoculation has replaced the production of the experimental disease in the monkey as the method of choice for the direct isolation and antigenic identification of virus from materials obtained from poliomyelitis patients.""' 12,16,18 In addition, tissue-culture methods have now made practical serologic tests for antibody development in man after natural poliomyelitis infection' '...